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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Helicos single molecule fluorescent sequencing ： ウィキペディア英語版 Helicos single molecule fluorescent sequencing The Helicos Genetic Analysis System platform was the first commercial NGS (Next Generation Sequencing) implementation to use the principle of single molecule fluorescent sequencing, a method of identifying the exact sequence of a piece of DNA. It was marketed by the now defunct Helicos Biosciences. The fragments of DNA molecules are first hybridized in place on disposable glass flow cells. Fluorescent nucleotides are then added one-by-one, with a terminating nucleotide used to pause the process until an image has been captured. From the image, one nucleotide from each DNA sequence can be determined. The fluorescent molecule is then cut away, and the process is repeated until the fragments have been completely sequenced.〔Thompson JF, Steinmann KE. 2010 Single Molecule Sequencing with a HeliScope Genetic Analysis System. Curr Protoc Mol Biol. Chapter 7:Unit7.10.〕 This sequencing method and equipment were used to sequence the genome of the M13 bacteriophage. ==Preparing the DNA== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Helicos single molecule fluorescent sequencing」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.